Global assembling of Academicians, Researchers, Scholars & Industry to disseminate and exchange information at 100+ Allied Academics Conferences

Islamic Azad University, Iran
Title: Detection and susceptibility pattern of biofilm-producing pseudomonas aeruginosa and using CRISPR/Cas systems to knock-out biofilm-specific antibiotic resistance genes
Biography

Muhammad Sadeqi Nezhad is majoring in Clinical Laboratory Science (BSc), Gorgan Islamic Azad University. He is a passionate, research-driven student looking to possess diversity of knowledge and necessary skills at Oncology/Pathology in medical school to begin a career in clinical research to discover diagnostic methods and treatment.

Abstract

Background: Biofilm plays an important role in chronic diseases and their eradication is very challengeable, when bacteria confront with antibiotics or strong immune system response they have the choice whether to be planktonic cells or form a biofilm, producing extra cell materials to enhance their survival. The aim of this study was the assessment of incidence and antibiotic algorithms of biofilm-producing Pseudomonas aeruginosa, an opportunistic pathogen and one of the most frequent causes of infectious disease in vulnerable patients. Methods: A total of 100 P.aeruginosa isolates were collected from five different clinical specimens and wards of the fifth Azar Hospital, Gorgan, Iran during November 2017. However, after isolating of samples under sterilized conditions, these strains have been identified as a P.aeruginosa through appropriate biochemical procedures and their antibiotic patterns according to NCCLS disk methodology have been examined; afterward, ELISA method was employed for the detection of biofilm producing P.aeruginosa. Results: Out of 100 clinical isolated P.aeruginosa, 31(31%) of them were biofilm producer. The frequency of biofilm positive strains among specimens have been observed; 56.2% from burned wounds, 36.4% from urines, 22.2% from respiratory secretions, 19.4% from blood cultures and 16.7% of the strains were biofilm positive from normal wound cultures ( P=0129). Besides, 50% of biofilm-producing P.aeruginosa were isolated in internal section followed by burned section (45.8%), ICU section (29.4%), surgical section (15.8%) and 9.2% in pediatric neurology section (P=0129). Furthermore, biofilm-producing P.aeruginosa indicated impressive resistance patterns to piperacillin (49.2%), Imipenem (49.2%), ciprofloxacin (47.6%), gentamicin (46.7%), ticarcillin (44.1%), cefepime (38.9%), ceftazidime (34.9%), ceftriaxone (34.3%), co-trimoxazole (34.1%) and cefotaxime (31.6%) respectively. Conclusion: This study demonstrated that there is a discrepancy in the outbreak of biofilm-producing P.aeruginosa among various specimens and also the pattern of antibiotic susceptibility and resistance did not follow a specific algorithm.

Speaker Presentations

Speaker Presentations

Speaker PDFs

Allied Academies Global Conference Directory

Mail us at

Program Enquiry
infectiousdiseases@alliedconferences.org
Sponsorship opportunity
infectiousdiseases@alliedconferences.org
General Enquiry
infectiousdiseases@alliedannualsummit.com
More details about sponsorship:sponsors@alliedacademies.com

Terms and Conditions

Responsibility

The organizers holds no responsibilities or liabilities of the personal articles of attendees at the venue against any kind of theft, lost, damage, due to any reason whatsoever. Delegates are entirely responsible for the safety of their own belongings.

 

Insurance

No insurance, of any kind, is included along with the registration in any of the events of the organization.

 

Transportation

Please note that transportation and parking is the responsibility of the registrant, Allied Academies will not be liable for any actions howsoever related to transportation and parking.

 

Press/Media

Press permission must be obtained from Allied Academies Conference Organizing Committee prior to the event. The press will not quote speakers or delegates unless they have obtained their approval in writing. The Allied Academies is an objective third-party non-profit organization and this conference is not associated with any commercial meeting company.

 

Requesting an Invitation Letter

For security purposes, letter of invitation will be sent only to those individuals who had registered for the conference after payment of complete registration fee. Once registration is complete, please contact infectiousdiseases@alliedconferences.org to request for a personalized letter of invitation, if not received until one month before the scheduled date of event.

All the bank charges applicable during refund will be deducted from the account of participant.

 

Cancellation Policy

All cancellations or modifications of registration must be made in writing to finance@alliedacademies.com

 

Postponement of event

If, due to any reason, Allied academies postpone an event and the participant is unable or unwilling to attend the conference on rescheduled dates, he/she is eligible for a credit of 100% of the registration fee paid. This credit shall only be used for another event organized by Allied academies within period of one year from the date of rescheduling.

 

Transfer of registration

All registrations, after payment of complete registration fee, are transferable to other persons from the same organization, if in case the person is unable to attend the event. Request for transfer of registration must be made by the registered person in writing to contact@alliedacademies.com . Details must include the full name of replaced new registrant, their title, contact phone number and email address. All other registration details will be assigned to the new person unless otherwise specified.

Registration can be transferred to one conference to another conference of Allied academies if the person is unable to attend one of conferences.

However, Registration cannot be transferred if intimated within 14 days of respective conference.

The transferred registrations will not be eligible for Refund.

This cancellation policy was last updated on April 04, 2015.

 

Visa Information

Keeping in view of increased security measures, we would like to request all the participants to apply for Visa as soon as possible.

Allied academies will not directly contact embassies and consulates on behalf of visa applicants. All delegates or invitees should apply for Business Visa only.

Important note for failed visa applications: Visa issues are not covered under the cancellation policy of Allied academies, including the inability to obtain a visa.

 

Refund Policy:

If the registrant is unable to attend, and is not in a position to transfer his/her participation to another person or event, then the following refund policies apply:

Keeping in view of advance payments towards Venue, Printing, Shipping, Hotels and other overhead charges, following Refund Policy Orders are available:

  • Before 60 days of the conference: Eligible for Full Refund after deduction of $100 towards service Fee.
  • Within 60-30 days of Conference: Eligible for 50% of payment Refund
  • Within 30 days of Conference: Not eligible for Refund
  • E-Poster Payments will not be refunded.

 

Accommodation Cancellation Policy:

Accommodation Service Providers (Hotels) have their own cancellation policies which are applicable when cancellations are made less than 30 days prior to arrival. If in case the registrant wishes to cancel or amend the accommodation, he/ she is expected to inform the organizing authorities on a prior basis. Allied academies will advise the registrant to ensure complete awareness about the cancellation policy of your accommodation provider, prior to cancellation or modification of their booking.

Copyright © 2018-2019 Allied Academies, All Rights Reserved.